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Do you have a protocol or suggested antibodies to carry out ICC staining on my completely differentiated ALI cultures?

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Move cytometry can be used to evaluate the efficiency of differentiation protocols with the STEMdiff™ system, by thinking about particular marker expression at Each and every stage with the differentiation procedure. It can even be used for other apps, including cell sorting, immunophenotyping, and purity evaluation.

It is important to quench the dissociation reagent utilizing FACS buffer or end medium, by introducing at the very least the exact same or double the quantity of the dissociation reagent.

Cautiously take away the supernatant without the need of disturbing the organoid pellet. Add 0.5 mL of ideal one-cell dissociation reagent into the tube. Particular incubation tips for various dissociation reagents are pointed out beneath:

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Likely again towards the plate, rinse Every single very well with one mL of FACS buffer and transfer the volume towards the 지방흡입 fifteen mL tube. Be aware: Retain cell suspension on ice soon after transfer into the tube until able Stem cell to run FACS.

Differentiation into adipocytes, osteoblasts, or chondrocytes with compatible MesenCult™ differentiation systems

After organoids are unveiled from Matrigel® and floating while in the harvesting Remedy, transfer every little thing into a fresh conical tube. Enable organoids settle by gravity or by centrifuging the tube for five minutes at 200 x g

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When introducing GCDR directly 가슴수술 to the cultures, pipette up and down several moments to mechanically crack up the dome completely in order that GCDR can act on cells for dissociation.

Notes: Use pipette ways to gently scratch the bottom of every perfectly so as to lift the monolayer off, then dissociate the cell clumps into one-cell suspension by pipetting up and down five - 10 instances.

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